Using this dilution calculator you can calculate how to dilute a stock solution for known concentration. All you have to do is to enter C1, C2 and V2 to calculate the required value of V1.

Calculate Dilution To make a Stock Solution

Enter C1, C2 & V2 to calculate V1.

Calculate now!

 

Concentration (start)

 

x

 

Volume (start)

 

=

 

Concentration (final)

 

x

 

Volume (final)

 

 

 

 

 

 

 

 

C1

 

V1

 

C2

 

V2

 


calculate
The Dilution Equation

This simple dilution calculator is based on equation mentioned below:

Concentration(start) x Volume(start) = Concentration(final) x Volume(final)

This equation is commonly abbreviated as: C1V1 = C2V2

Here is an example of dilution calculation using the simple dilution calculator

In chemistry, particularly biochemistry, dilution calculations are pretty common. Despite being simple dilution concentration calculation is particularly difficult for majority of the students. Efforts have been made this calculator as simple as possible. Consider the following sample:

Following calculation is to help you find volume of a given stock solution of 20 mM in order to develop 40ml of a 100 μM solution.

Using the equation C1V1 = C2V2, where C1=20 mM, C2=100 μM, V2=40 ml and V1 is to know:

  1. Type 20 into the Concentration (C1) and select (millimolar)
  2. Type 100 into the Concentration (C2) and select (micromolar)
  3. Type 40 into the Volume (V2) and select (milliliter)
  4. Click calculate
  5. The answer of 200 microliter (0.2 ml) appears in the Volume (V1), simple!

 

This a relatively a simple dilution calculation. The results varies from solution to solution. Since all problems are a bit different from each other so it makes dilution calculations a little difficult. Generally what students think that by memorizing a single equation their all dilution problems will be solved, but sorry to say it can be proved wrong at many of times in your experiments since there are not always one type of stock solutions. Here are three simple rules if you can remember them I am sure chances of errors will be lessen.

  • Rule #1: The concentration of a solution is the amount of solute in the solution divided by the total volume of the solution. This rule is used for volume based concentration units.
  • Rule #2: When a solution is diluted, the amount of solute in solution remains constant. This is a statement of the law of conservation of matter.
  • Rule #3: A sample of a solution has the same concentration as the original solution. This rule has been derived from the definition of a solution (a liquid is a homogeneous mixture).

The above mentioned approach to volume or concentration calculations is more flexible as compare to learning dilution equations as the rules are based on fundamentals of chemistry rather than simple a*b*c. Adapting to these rules will lead you to get better understanding with your stock solution and the process involved.

According to Rule #1, the the formula for calculating concentration is mentioned below. The same dilution formula can be used to calculate Volume and Amount.

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Hope it was useful. We are planning to add more calculators including Molarity Calculator, Serial Dilution Calculator and other Chemical Calculators to make it east for students just like us.

Please share you suggestions and thoughts with us.